- Whole genome nucleotide sequence analysis of a range of T. parva isolates with a focus on the three component stocks that comprise the “Muguga” cocktail. This will complement efforts by ILRI to define the composition of infection and treatment (ITM) sporozoite stabilates.
- Evaluation of sequence polymorphisms of genes encoding proteins recognised by cytotoxic T cells induced by infection and treatment method.
- Genome wide profiling of genetic variation by comparing sequence data to the published reference genome. This will focus on a collection of T. parva isolates of buffalo origin isolated from a single farm in Kenya. Results will allow for identification of conserved and rapidly evolving genes and preliminary analysis of linkage disequilibrium from a specific T.parva sub-population in the field.
- Identification of all sites of physical recombination using the parents and progeny of an experimental cross between two T. parva isolates.
- Building scientific capacity in the East African region through training of a Kenyan bio-informatician in advanced bioinformatics techniques at the bio-informatics institute at ETH.
- Whole genome sequences of a range of isolates of T. parva from cattle and buffalo
- Identification of highly conserved and fast evolving genes in T. parva isolates from cattle and buffalo
- Identfication of genes that are likely to be under positive selection and therefore related to host parasite interactions
- Analysis of sequence polymorphism of proteins known to be targets of cytotoxic T cells.
- Identification of SNP for micro-epidemiological studies
- Analysis of these data will also contribute to the understanding of the biology of other Apicomplexan parasites including Plasmodium falciparum, the causative agent of human malaria